Sensitive, Simple and Rapid Spectrophotometric Method for Monitoring Treatment Concentration of Chloramine-T on Fish Culture Facilities and Environmental Water Samples using Green Analytical Reagent
Riyad Ahmed Al-Okab1,* and Amal Mohammed Al-Awadhi2
1,*Chemistry Department, Faculty of Applied Science, Taiz University, Taiz, Republic of Yemen.
2Medical Sciences Dean’s Assistant, Lebanese International University, Sana’a, Republic of Yemen.
When studying the effectiveness of the drug for controlling bacterial gill disease. N-sodium-N-chloro-rho-toluenesulfonamide (chloramine-T) concentration must be monitored during treatments of fish. A simple, sensitive and rapid spectrophotometric method, using phenoxazine Cisapride (CSP) and Metaclopramide as green analytical reagents for determination of chloramine-T (CAT) was proposed. The methods are based on the oxidation of Cisapride (CSP) and Metaclopramide (MCP) by chloramine-T in acidic media and coupling with phenoxazine to produce red colour product that has maximum absorption at 520 nm. The calibration curves are linear over 0.50 – 4.50 µg mL‑1 and 0.45 – 4.00 µg mL‑1 respectively. The concentration measurements are reproducible within a relative standard deviation of 1% and recoveries are 98.0 -102.0 %. The performance of proposed methods were evaluated in terms of Student’s t-test and Variance ratio F-test that indicate the significance of proposed methods over the standard spectrophotometric method. The proposed method was successfully applied in the determination of chloramine-T in fish culture facilities and environmental water samples.
Keywords: Chloramine-T, Spectrophotometric method, Fish Culture Facilities, Phenoxazine cisapride, green analytical reagents.
